Usually not. When I've reassembled crankcases on motorbikes, the bond / sealer for the mating faces (which have no gasket) just needs a couple of minutes to 'flash off' and go tacky, then the cases can be fitted together, torqued up and filled with oil / fired up immediately.
I usually use a very thin brushing of Wellseal on joints with gaskets (paper, card or metal) and that just needs a minute or so to tack up. It helps with sealing and also makes the joint easier to disassemble if it ever needs to come apart, as it stops the gasket adhering to the joint faces.
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